IOI BLAST Web App

Search nucleotide query sequences against an uploaded nucleotide sample set using NCBI BLAST+, then align the best matching regions with MAFFT. See an example output here.

Implemented tools and sources

NCBI BLAST+ using blastn and makeblastdb: blastn: 2.17.0+
MAFFT for multiple sequence alignment: v7.526 (2024/Apr/26)

Submit your job

STEP 1. Provide a nucleotide query sequence

Paste query sequence

Or upload query FASTA

Drop query FASTA here or click to select

STEP 2. Upload the nucleotide sample set database or genome inputs

Database FASTA, genome FASTA(s), or ZIP archive of genomes

Drop database FASTA or genome files here or click to select

STEP 3. Choose BLAST and alignment settings

Max target sequences

E-value threshold

Word size

BLAST threads

Alignment hit limit

Ineos Oxford Institute for antimicrobial research

University of Oxford

Sir William Dunn School of Pathology

South Parks Road

Oxford, OX1 3RE

United Kingdom